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Isolation of a new restriction enzyme, ApaCI, an isoschizomer of BamHI produced by Acetobacter pasteurianus.

作者信息

Grones J, Turna J

机构信息

Department of Biochemistry, Faculty of Science, Comenius University, Bratislava, Czechoslovakia.

出版信息

Folia Microbiol (Praha). 1992;37(5):353-6. doi: 10.1007/BF02815661.

DOI:10.1007/BF02815661
PMID:1493903
Abstract

A new Type II restriction endonuclease ApaCI purified from Acetobacter pasteurianus is an isoschizomer of BamHI that cleaves at the nucleotide sequence 5'-G/GATCC-3' of double-stranded DNA. The single restriction activity present in this strain permits rapidly purified 30,000 units of cleavage activity from 10 g of freshly harvested cells. The resulting ApaCI preparation is free of contaminant nuclease activities that might interfere with in vitro manipulation of DNA.

摘要

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