Some properties of restriction endonuclease ApaBI from Acetobacter pasteurianus.

A new site-specific endonuclease has been isolated from Acetobacter pasteurianus and has been named ApaBI. The enzyme recognizes 35 cleavage sites on bacteriophage lambda DNA, 20 sites on adenovirus-2 DNA and 2 sites on plasmid pBR322. The recognition sequence for this enzyme is 3'-CGT/NNNNNACG-5' 5'-GCANNNNN/TGC-3'.