[洗涤剂以及钠钾ATP酶和镁ATP酶的抑制对红细胞膜巯基的影响]

[Influence of detergents and inhibition of Na+,K+-ATPase and Mg2+ATPase on erythrocyte membrane SH-groups].

作者信息

Megalinskaia A P, Skobets E M, Lishko V K

出版信息

Ukr Biokhim Zh (1978). 1978 Mar-Apr;50(2):222-5.

Abstract

The article deals with the influence of sodium dodensylsulfate, lubrol and also of inhibition of Na+,K+-ATPase and Mg2+-ATPase activities by N-ethyl malemid on the form of curves of erythrocyte membranes amperometric titration by Ag+. It is shown that changes in a conformational structure of proteins membranes under the influence of detergents results in the transformation of SH-group of type I to SH-groups of type II. A 70% fall of Na+,K+-ATPase activity has the same result. So, the form of the curves for titration of erythrocyte membranes sulphydryl groups may serve as an indicator to the morpholocial and functional state of the membranes protein part.

摘要

本文探讨了十二烷基硫酸钠、卢勃罗尔以及N - 乙基马来酰胺对Na⁺,K⁺ - ATP酶和Mg²⁺ - ATP酶活性的抑制作用对红细胞膜银离子安培滴定曲线形式的影响。结果表明，在去污剂的作用下，蛋白质膜构象结构的变化导致I型巯基转变为II型巯基。Na⁺,K⁺ - ATP酶活性下降70%也会产生相同的结果。因此，红细胞膜巯基滴定曲线的形式可作为膜蛋白部分形态和功能状态的指标。