Ambrosio S, De Oliveira J R, Bartrons R
Unitat de Bioquímica, Facultat Odontologia, Universitat de Barcelona, Spain.
J Biochem Biophys Methods. 1992 Dec;25(4):237-44. doi: 10.1016/0165-022x(92)90018-6.
Galactosamine is quickly metabolized to galactosamine 1-phosphate in rats treated with this compound. An HPLC method to quantify hexosamine phosphates in biological samples is described, modified from the o-phthaldialdehyde amino acid analysis procedure. o-Phthaldialdehyde derivatives of hexosamines and hexosamine-phosphates can be eluted from a reverse-phase column at different retention times, with a total analysis time of 30 min and without overlapping with free amino acids at physiological concentrations. The standard curves are linear between 1 and 40 nmol. This simple method is more selective and sensitive than previous enzymatic analyses of hexosamine phosphorylation.
