一种用于估算己糖胺的高效液相色谱法的开发及其在测定大鼠和人胃黏膜中黏蛋白含量的应用。

Development of an HPLC method to estimate hexosamines and its application to determine mucin content in rat and human gastric mucosa.

Ishihara K, Kameyama J, Hotta K

Department of Chemistry and Biochemistry, School of Medicine, Kitasato University, Sagamihara, Japan.

Comp Biochem Physiol B. 1993 Apr;104(4):781-6. doi: 10.1016/0305-0491(93)90213-o.

Abstract

An HPLC analysis of hexosamines and related compounds modified from the Waters Pico-Tag amino acid analysis procedure is described. 2. This method is applicable to determine glucosamine and galactosamine not only in the hydrolyzate of purified glycoconjugate such as gastric mucin and ovalbumin but also in crude materials such as rat gastric mucosal extract and powdered bovine nasal cartilage. 3. Compared with a conventional colorimetric hexosamine determination, sensitivity of this method is almost 100 times higher. 4. By applying this method, a simple but highly sensitive procedure to determine mucin content in a crude stomach specimen of rat and that in a biopsied human gastric mucosa was also developed.

摘要

描述了一种基于沃特世Pico-Tag氨基酸分析方法改进的用于己糖胺及相关化合物的高效液相色谱分析方法。2. 该方法不仅适用于测定纯化糖缀合物（如胃粘蛋白和卵清蛋白）水解产物中的葡萄糖胺和半乳糖胺，也适用于大鼠胃黏膜提取物和牛鼻软骨粉等粗提物。3. 与传统的比色法测定己糖胺相比，该方法的灵敏度几乎高100倍。4. 通过应用该方法，还开发了一种简单但高度灵敏的程序，用于测定大鼠粗胃标本和人胃黏膜活检标本中的粘蛋白含量。