Lepistö J, Laato M, Niinikoski J, Lundberg C, Gerdin B, Heldin C H
Department of Surgery, University of Turku, Finland.
J Surg Res. 1992 Dec;53(6):596-601. doi: 10.1016/0022-4804(92)90260-7.
Platelet-derived growth factor (PDGF) has been suggested to have a significant role in wound healing. The present work was aimed at studying the effects of PDGF-AA and PDGF-BB homodimers on developing granulation tissue in rats. Subcutaneously implanted hollow cylindrical cellulose sponges were used as an inductive matrix for the ingrowth of granulation tissue. Fifty microliters of solutions containing 0, 5, 50, or 500 ng of PDGF-AA or PDGF-BB homodimers was injected daily into the sponges; 7 days after implantation the granulation tissue in the sponge cylinders was analyzed. Five hundred nanograms of PDGF-BB stimulated significantly the accumulation of collagen, indicated by the elevated hydroxyproline content of the sponge (+34%, P < 0.001). Similarly, the amounts of RNA-ribose, nitrogen, hexosamines, and uronic acids were significantly higher, reflecting a PDGF-BB-induced increase in the accumulation of RNA, protein, and glycosaminoglycans. Analyses of wound fluid showed no essential changes in the composition of different cell types after PDGF-BB-treatment. The PDGF-AA-treatment increased significantly the mean amount of RNA but there were no significant changes in other parameters. In vitro both PDGF-AA and PDGF-BB increased significantly the number of rat granulation tissue derived fibroblasts in culture at concentrations of 10 and 30 ng/ml. This proliferative effect resulted in a lowered level of protein synthesis per cell. To conclude, PDGF-BB accelerates granulation tissue formation both in vitro and in vivo, whereas PDGF-AA is effective in vitro but it is clearly less effective in vivo.
血小板衍生生长因子（PDGF）已被认为在伤口愈合中起重要作用。本研究旨在探讨PDGF-AA和PDGF-BB同二聚体对大鼠肉芽组织形成的影响。皮下植入的中空圆柱形纤维素海绵用作肉芽组织向内生长的诱导基质。每天向海绵中注射50微升含有0、5、50或500纳克PDGF-AA或PDGF-BB同二聚体的溶液；植入7天后，分析海绵圆柱体内的肉芽组织。500纳克的PDGF-BB显著刺激了胶原蛋白的积累，海绵中羟脯氨酸含量升高表明了这一点（+34%，P<0.001）。同样，RNA-核糖、氮、己糖胺和糖醛酸的含量也显著更高，反映出PDGF-BB诱导的RNA、蛋白质和糖胺聚糖积累增加。伤口液分析显示，PDGF-BB处理后不同细胞类型的组成没有本质变化。PDGF-AA处理显著增加了RNA的平均含量，但其他参数没有显著变化。在体外，PDGF-AA和PDGF-BB在浓度为10和30纳克/毫升时均显著增加了培养的大鼠肉芽组织来源的成纤维细胞数量。这种增殖效应导致每个细胞的蛋白质合成水平降低。总之，PDGF-BB在体外和体内均加速肉芽组织形成，而PDGF-AA在体外有效，但在体内明显效果较差。
