Knorr M, Völker M, Denk P O, Wunderlich K, Thiel H J
University Eye Clinic Tübingen, Department of General Ophthalmology, Germany.
Graefes Arch Clin Exp Ophthalmol. 1997 Oct;235(10):667-71. doi: 10.1007/BF00946945.
Although platelet-derived growth factor (PDGF) has been thought to be critical in the wound-healing response of Tenon's capsule fibroblasts after glaucoma filtration surgery, no information is currently available concerning the proliferative effect of PDGF isoforms on this cell type. The aim of the present study was to evaluate the proliferative effect of PDGF-AB heterodimer and PDGF-AA and -BB homodimers on cultured human Tenon's capsule fibroblasts.
Human Tenon's capsule fibroblasts, cultured under serum-free conditions, were stimulated with PDGF-AA, -AB and -BB isoforms in concentrations ranging from 1 to 100 ng/ml. Cell numbers were determined on days 1, 3, 5 and 7, using a cell counter.
Addition of PDGF-AB and -BB led to a dose-dependent increase in cell proliferation. A maximal response (79.9% over control) was obtained after 7 days with 30 ng/ml of PDGF-BB, with an EC50 of 8.9 ng/ml. The maximal increase in cell proliferation caused by PDGF-AB (30 ng/ml) was 54.9%, with an EC50 of 12.5 ng/ml. Stimulation with PDGF-AA revealed a significant effect only with concentrations higher than 30 ng/ml.
Our results indicate that PDGF-AB and -BB isoforms are potent stimulators of proliferation of human Tenon's capsule fibroblasts, suggesting that PDGF-AB and -BB isoforms play an important role in the wound-healing response after glaucoma filtration surgery.
尽管血小板衍生生长因子（PDGF）被认为在青光眼滤过手术后Tenon囊成纤维细胞的伤口愈合反应中起关键作用，但目前尚无关于PDGF亚型对该细胞类型增殖作用的信息。本研究的目的是评估PDGF-AB异二聚体以及PDGF-AA和-BB同二聚体对培养的人Tenon囊成纤维细胞的增殖作用。
在无血清条件下培养的人Tenon囊成纤维细胞，用浓度范围为1至100 ng/ml的PDGF-AA、-AB和-BB亚型进行刺激。使用细胞计数器在第1、3、5和7天测定细胞数量。
添加PDGF-AB和-BB导致细胞增殖呈剂量依赖性增加。用30 ng/ml的PDGF-BB处理7天后获得最大反应（比对照增加79.9%），半数有效浓度（EC50）为8.9 ng/ml。PDGF-AB（30 ng/ml）引起的细胞增殖最大增加为54.9%，EC50为12.5 ng/ml。仅在浓度高于30 ng/ml时，PDGF-AA刺激才显示出显著作用。
我们的结果表明，PDGF-AB和-BB亚型是人类Tenon囊成纤维细胞增殖的有效刺激剂，提示PDGF-AB和-BB亚型在青光眼滤过手术后的伤口愈合反应中起重要作用。
