原代大鼠肝实质细胞与肝癌细胞融合建立的杂交细胞系中的外源物代谢酶活性。

Xenobiotic-metabolizing enzyme activities in hybrid cell lines established by fusion of primary rat liver parenchymal cells with hepatoma cells.

Utesch D, Arand M, Thomas H, Petzinger E, Oesch F

Institute of Toxicology, University of Mainz, Germany.

Xenobiotica. 1992 Dec;22(12):1451-7. doi: 10.3109/00498259209056695.

Abstract

The activities of xenobiotic-metabolizing enzymes were determined in hybrid cell lines (hepatocytoma, HPCT) which have been established by fusion of liver parenchymal cells from adult rat (PC) with cells from a Reuber hepatoma cell line (FAO). 2. Cytochrome P450 was not measurable spectrophotometrically in FAO and HPCT. P450-dependent conversion of testosterone was below the detection limit in FAO and only marginally present in HPCT. 3. Microsomal and cytosolic epoxide hydrolase, glutathione S-transferase and phenol sulphotranserase were low or even below detection limit in FAO. These enzyme activities were significantly higher in HPCT and correspond to about 1-10% the activities measured in PC. 4. 1-Naphthol UPD-glucuronosyl transferase activity was about 20% in FAO and about 100% in HPCT compared to PC. 5. Metabolic conversion of benzo[a]pyrene was low in FAO, high in PC, and intermediate in HPCT. The presented data, however, do not allow the conclusion whether this intermediate rate is catalyzed by similar P450 isoenzymes as in PC. 6. Due to the easily measurable phase II-metabolizing enzyme activities HPCT may, however, be useful for in vitro enzyme induction or repression studies.

摘要

在通过将成年大鼠肝实质细胞（PC）与鲁伯肝癌细胞系（FAO）的细胞融合而建立的杂交细胞系（肝癌细胞系，HPCT）中测定了异生物质代谢酶的活性。2. 在FAO和HPCT中，用分光光度法无法检测到细胞色素P450。FAO中睾酮的P450依赖性转化低于检测限，在HPCT中仅微量存在。3. FAO中的微粒体和胞质环氧化物水解酶、谷胱甘肽S-转移酶和酚磺基转移酶活性较低，甚至低于检测限。这些酶活性在HPCT中显著更高，约相当于PC中测得活性的1%-10%。4. 与PC相比，FAO中1-萘酚UDP-葡萄糖醛酸基转移酶活性约为20%，HPCT中约为100%。5. 苯并[a]芘在FAO中的代谢转化率较低，在PC中较高，在HPCT中处于中间水平。然而，所提供的数据无法得出该中间速率是否由与PC中相似的P450同工酶催化的结论。6. 由于HPCT中易于测量的II相代谢酶活性，它可能对体外酶诱导或抑制研究有用。