Krupski G, Kiefer F, Wiebel F J
Xenobiotica. 1985 Aug-Sep;15(8-9):781-7. doi: 10.3109/00498258509047441.
The activity of various xenobiotic-metabolizing enzymes was examined throughout the growth cycle (16d) of the well-differentiated rat hepatoma cell line C2Rev7. Cytochrome P-450-dependent aldrin epoxidase activity showed a peak on day 3 after plating of cells and decreased by more than 90% during the following six days. Glutathione S-transferase and UDP-glucuronosyl transferase with 4-hydroxybiphenyl as substrate also showed decreases in their activities towards the later phase of the growth cycle, although to lesser extents than the mono-oxygenase. The activity of cytochrome c reductase and of the UDP-glucuronosyl transferase with 3-hydroxybenzo[a]pyrene as substrate remained constant throughout the growth cycle. Aldrin epoxidase activities varied markedly with the number of cells plated. The results suggest that the balance of activating and inactivating pathways may vary considerably during the growth cycle of differentiated hepatoma cells. This should be taken into account when standardizing these cells as test systems for the assessment of cytotoxic and genotoxic chemicals.
在高分化大鼠肝癌细胞系C2Rev7的整个生长周期（16天）中，检测了各种外源生物代谢酶的活性。细胞色素P - 450依赖的艾氏剂环氧化酶活性在细胞接种后第3天出现峰值，并在接下来的6天内下降超过90%。以4 - 羟基联苯为底物的谷胱甘肽S - 转移酶和UDP - 葡萄糖醛酸基转移酶在生长周期后期其活性也有所下降，尽管下降程度比单加氧酶小。细胞色素c还原酶以及以3 - 羟基苯并[a]芘为底物的UDP - 葡萄糖醛酸基转移酶的活性在整个生长周期中保持恒定。艾氏剂环氧化酶活性随接种细胞数量的变化显著。结果表明，在分化的肝癌细胞生长周期中，激活和失活途径的平衡可能有很大差异。在将这些细胞标准化作为评估细胞毒性和遗传毒性化学物质的测试系统时，应考虑到这一点。
