定量成年猫蚤（蚤目：蚤科）粪便的分光光度法。

Spectrophotometric method of quantifying adult cat flea (Siphonaptera: Pulicidae) feces.

作者信息

Kern W H, Koehler P G, Patterson R S, Wadleigh R W

机构信息

Department of Entomology and Nematology, University of Florida, Gainesville 32611.

出版信息

J Med Entomol. 1992 Mar;29(2):221-5. doi: 10.1093/jmedent/29.2.221.

DOI:10.1093/jmedent/29.2.221

PMID:1495033

Abstract

Cat flea, Ctenocephalides felis felis (Bouché), feces are an essential part of larval nutrition under natural conditions. The mass values of adult flea feces can be measured by dissolving samples of flea feces in Drabkin's reagent, filtering, centrifuging, and measuring absorbance spectrophotometrically at 540 nm. Either flea feces or air-dried host blood can be used to generate the standard curve used to convert absorbance (optical density) values into mass values. Debris collected from flea-infested house cats averaged 23.02 mg debris per cat per h with an average of 10.41 mg flea feces per cat per h. Flea feces deposited in the environment serve as potential larval food. Adult flea feces comprised an average of 44.28% of the debris deposited from infested domestic house cats in this study.

摘要

猫蚤，即猫栉首蚤指名亚种（布歇），其粪便在自然条件下是幼虫营养的重要组成部分。成年跳蚤粪便的质量值可通过将跳蚤粪便样本溶解于德拉布金试剂、过滤、离心，并在540纳米处用分光光度计测量吸光度来测定。跳蚤粪便或风干的宿主血液均可用于生成标准曲线，该曲线用于将吸光度（光密度）值转换为质量值。从感染跳蚤的家猫身上收集的碎片平均每只猫每小时为23.02毫克，平均每只猫每小时有10.41毫克跳蚤粪便。沉积在环境中的跳蚤粪便可作为潜在的幼虫食物。在本研究中，成年跳蚤粪便平均占感染家猫所沉积碎片的44.28%。