Jaworski D C, Rosell R, Coons L B, Needham G R
Department of Entomology, Ohio State University, Columbus 43210-1292.
J Med Entomol. 1992 Mar;29(2):305-9. doi: 10.1093/jmedent/29.2.305.
A specific antiserum (12C) raised to a 90-kDa immunogenic component of salivary glands of the tick Rhipicephalus appendiculatus recognized similar 90-kDa polypeptides from salivary glands of the American dog tick, Dermacentor variabilis, and the lone star tick, Amblyomma americanum, as well as 70-kDa polypeptides in the cement of D. variabilis, A. americanum, and R. sanguineus (brown dog tick). The reduction in size of the polypeptide for these ticks suggests that it is modified in some way during or after secretion. Immunostaining of salivary glands of unfed- and partially-fed female D. variabilis localized an immunoreactive protein in the d- and e-cells of the type III acini. The quantity of label in granules of glands from unfed ticks was visibly greater than in the granules of glands from partially fed ticks, suggesting that this component is secreted within the first 2 d of feeding. Collectively, these data support the conclusion that a 90-kDa polypeptide of saliva is conserved among ixodid tick genera and is a component of the attachment cement.
一种针对微小扇头蜱唾液腺90 kDa免疫原性成分制备的特异性抗血清（12C），可识别来自美洲犬蜱、变异革蜱和美洲钝眼蜱唾液腺中的类似90 kDa多肽，以及变异革蜱、美洲钝眼蜱和血红扇头蜱（棕狗蜱）的附着胶中的70 kDa多肽。这些蜱类多肽大小的减小表明其在分泌过程中或分泌后以某种方式发生了修饰。对未进食和部分进食的雌性变异革蜱唾液腺进行免疫染色，在III型腺泡的d细胞和e细胞中定位到一种免疫反应性蛋白。未进食蜱类腺体颗粒中的标记量明显大于部分进食蜱类腺体颗粒中的标记量，这表明该成分在进食的头2天内分泌。总体而言，这些数据支持以下结论：唾液中的90 kDa多肽在硬蜱属中是保守的，并且是附着胶的一个成分。
