Slovák M, Hajnická V, Labuda M, Fuchsberger N
Institute of Zoology, Slovak Academy of Sciences, Bratislava.
Folia Parasitol (Praha). 2000;47(1):67-71. doi: 10.14411/fp.2000.013.
Salivary gland extracts (SGE) from unfed and 5 days fed adult female Ixodes ricinus (Linnaeus, 1758); Haemaphysalis inermis (Birula, 1895) and Dermacentor reticulatus (Fabricius, 1794) ticks were prepared. The protein content after feeding increased by 10.6, 8.7 and 6.8 times, respectively. Extracts were equilibrated to the same protein content and submitted to SDS-polyacrylamide gel electrophoresis followed by computer analysis of the scanned gels. Relative differences in protein profiles of extracts obtained from unfed and partially fed ticks were found in all species and some of them were similar in all three species used in the study. Results demonstrate that the increase of the protein content in salivary glands during the feeding does not occur proportionally. Some proteins are synthesised preferentially (67.1 kDa, 13.5 kDa) but other bands (in range of 15-16 kDa) present in the SGE derived from unfed ticks are less discernible in that of fed ticks.
制备了未进食和进食5天的成年雌性蓖麻硬蜱（林奈，1758年）、无盾血蜱（比鲁拉，1895年）和网纹革蜱（法布里修斯，1794年）的唾液腺提取物（SGE）。进食后蛋白质含量分别增加了10.6倍、8.7倍和6.8倍。将提取物平衡至相同的蛋白质含量，然后进行SDS聚丙烯酰胺凝胶电泳，随后对扫描的凝胶进行计算机分析。在所有物种中均发现了从未进食和部分进食蜱虫获得的提取物蛋白质谱的相对差异，其中一些差异在研究中使用的所有三个物种中相似。结果表明，进食期间唾液腺中蛋白质含量的增加并非成比例发生。一些蛋白质优先合成（67.1 kDa、13.5 kDa），但未进食蜱虫的SGE中存在的其他条带（在15 - 16 kDa范围内）在进食蜱虫的SGE中较难辨别。
