GIRARDI A J, ALLEN E G, SIGEL M M
J Exp Med. 1952 Sep;96(3):233-46. doi: 10.1084/jem.96.3.233.
The pattern of growth of meningopneumonitis virus in vitro seemed to be similar to that occurring in ovo and thus the initial stages of development, the adsorption and the latent periods, were investigated by the use of tissue culture procedures. The initial increment of infectivity in allantoic membrane suspensions following virus inoculation in ovo was due to prolonged adsorption of virus and not to immediate virus reproduction. The length of the adsorption period varied with the virus dilution employed. The reduction of virus titer in allantoic membrane suspensions subsequent to adsorption was due to a change of infectious virus to a non-infectious form and this seemed to be a part of the normal developmental cycle of the virus. The possible causes for both prolonged virus adsorption and for the subsequent development of a non-infectious form are discussed.
脑膜炎肺炎病毒在体外的生长模式似乎与在鸡胚内的生长模式相似，因此利用组织培养程序对其发育的初始阶段、吸附和潜伏期进行了研究。在鸡胚中接种病毒后，尿囊膜悬液中感染性的初始增加是由于病毒的长时间吸附，而非病毒的立即繁殖。吸附期的长短随所用病毒稀释度而变化。吸附后尿囊膜悬液中病毒滴度的降低是由于感染性病毒转变为非感染性形式，这似乎是病毒正常发育周期的一部分。文中讨论了病毒长时间吸附以及随后形成非感染性形式的可能原因。
