Lu H, Zawel L, Fisher L, Egly J M, Reinberg D
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635.
Nature. 1992 Aug 20;358(6388):641-5. doi: 10.1038/358641a0.
Phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II is believed to control the transition from transcription initiation to elongation. The general transcription factor IIH (TFIIH) contains a kinase activity capable of phosphorylating this domain. Factors that promote the association of RNA polymerase II with the preinitiation complex stimulate this activity. The transcription factor IIE, which is required for the stable association of TFIIH with the preinitiation complex, affects the processivity of TFIIH kinase.
人们认为，RNA聚合酶II最大亚基的羧基末端结构域的磷酸化作用可控制从转录起始到延伸的转变。通用转录因子IIH（TFIIH）具有能够使该结构域磷酸化的激酶活性。促进RNA聚合酶II与起始前复合物结合的因子会刺激这种活性。转录因子IIE是TFIIH与起始前复合物稳定结合所必需的，它会影响TFIIH激酶的持续合成能力。
