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一种编码RAP74的互补DNA，RAP74是RNA聚合酶II转录的通用起始因子。

A cDNA encoding RAP74, a general initiation factor for transcription by RNA polymerase II.

作者信息

Finkelstein A, Kostrub C F, Li J, Chavez D P, Wang B Q, Fang S M, Greenblatt J, Burton Z F

机构信息

Department of Biochemistry, Michigan State University, E. Lansing 48824.

出版信息

Nature. 1992 Jan 30;355(6359):464-7. doi: 10.1038/355464a0.

DOI:10.1038/355464a0
PMID:1734284
Abstract

RAP30/74 (also known as TFIIF, beta gamma and FC is one of several general factors required for initiation by RNA polymerase II. The small RAP30 subunit of RAP30/74 binds directly to polymerase and appears structurally and functionally homologous to bacterial sigma factors in their RNA polymerase-binding region. RAP30/74 or recombinant RAP30 suppresses nonspecific binding of RNA polymerase II to DNA and is required for RNA polymerase II to assemble stably into a preinitiation complex containing promoter DNA and the general factors TFIID, TFIIA and TFIIB; both RAP30 and RAP74 are physical components of the preinitiation complex. A complementary DNA encoding human RAP30 has been isolated, and here we report the isolation of a cDNA encoding human RAP74. RAP30 and RAP74 produced in Escherichia coli can be used in place of natural human RAP30/74 to direct accurate transcription initiation by RNA polymerase II in vitro.

摘要

RAP30/74（也称为TFIIF、βγ和FC）是RNA聚合酶II起始转录所需的几种通用因子之一。RAP30/74的小RAP30亚基直接与聚合酶结合，并且在其RNA聚合酶结合区域在结构和功能上似乎与细菌的σ因子同源。RAP30/74或重组RAP30可抑制RNA聚合酶II与DNA的非特异性结合，并且是RNA聚合酶II稳定组装成包含启动子DNA以及通用因子TFIID、TFIIA和TFIIB的起始前复合物所必需的；RAP30和RAP74都是起始前复合物的物理组成部分。已分离出编码人RAP30的互补DNA，在此我们报告编码人RAP74的cDNA的分离。在大肠杆菌中产生的RAP30和RAP74可用于替代天然人RAP30/74，以在体外指导RNA聚合酶II进行准确的转录起始。

相似文献

1
A cDNA encoding RAP74, a general initiation factor for transcription by RNA polymerase II.一种编码RAP74的互补DNA，RAP74是RNA聚合酶II转录的通用起始因子。
Nature. 1992 Jan 30;355(6359):464-7. doi: 10.1038/355464a0.
2
Characterization of cDNA for the large subunit of the transcription initiation factor TFIIF.转录起始因子TFIIF大亚基的cDNA特性分析
Nature. 1992 Jan 30;355(6359):461-4. doi: 10.1038/355461a0.
3
Production of human RAP30 and RAP74 in bacterial cells.在细菌细胞中生产人源RAP30和RAP74。
Protein Expr Purif. 1993 Jun;4(3):207-14. doi: 10.1006/prep.1993.1027.
4
Importance of codon preference for production of human RAP74 and reconstitution of the RAP30/74 complex.密码子偏好性对人RAP74产生及RAP30/74复合物重组的重要性。
Protein Expr Purif. 1994 Oct;5(5):476-85. doi: 10.1006/prep.1994.1067.
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Conserved sequence motifs in the small subunit of human general transcription factor TFIIE.人类通用转录因子TFIIE小亚基中的保守序列基序。
Nature. 1991 Dec 5;354(6352):401-4. doi: 10.1038/354401a0.
6
RAP30/74 (transcription factor IIF) is required for promoter escape by RNA polymerase II.RAP30/74（转录因子IIF）是RNA聚合酶II启动子逃逸所必需的。
J Biol Chem. 1993 Sep 25;268(27):20482-9.
7
The general transcription factor RAP30 binds to RNA polymerase II and prevents it from binding nonspecifically to DNA.通用转录因子RAP30与RNA聚合酶II结合，并阻止其非特异性地与DNA结合。
Mol Cell Biol. 1992 Jan;12(1):30-7. doi: 10.1128/mcb.12.1.30-37.1992.
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Roles for both the RAP30 and RAP74 subunits of transcription factor IIF in transcription initiation and elongation by RNA polymerase II.转录因子IIF的RAP30和RAP74亚基在RNA聚合酶II转录起始和延伸中的作用。
J Biol Chem. 1994 Oct 14;269(41):25684-91.
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Dissection of transcription factor TFIIF functional domains required for initiation and elongation.转录起始和延伸所需转录因子TFIIF功能结构域的剖析。
Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):6042-6. doi: 10.1073/pnas.92.13.6042.
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Distinct domains of hTAFII100 are required for functional interaction with transcription factor TFIIF beta (RAP30) and incorporation into the TFIID complex.hTAFII100的不同结构域对于与转录因子TFIIFβ（RAP30）的功能相互作用以及并入TFIID复合物是必需的。
EMBO J. 1996 Jul 15;15(14):3702-12.

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