Finch R A, Chan P K
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030.
Oncology. 1992;49(3):223-6. doi: 10.1159/000227043.
Nucleophosmin/B23 is a nucleolar phosphoprotein which redistributes from nucleoli to nucleoplasm (B23-translocation) when cells are exposed to certain anticancer drugs, particularly intercalators. The B23-translocation assay has been demonstrated in cell culture to correlate with drug effects and to detect drug-resistant cells. We now report the effect of actinomycin D on B23-translocation in P388D1 cells implanted in DBA/2 mice. B23-translocation was observed in cells after actinomycin D treatment in a dosage- and time-dependent manner. Translocation could be observed within 30 min after drug treatment. Complete B23-translocation with at least 1-day duration was achieved by a single injection of 0.25 mg/kg. Reduced dosages produced partial B23-translocation with shorter durations. These results indicate that B23-translocation may be useful in monitoring drug effects in animals.
核磷蛋白/B23是一种核仁磷蛋白，当细胞暴露于某些抗癌药物，特别是嵌入剂时，它会从核仁重新分布到核质（B23易位）。在细胞培养中已证明B23易位试验与药物作用相关，并能检测耐药细胞。我们现在报告放线菌素D对植入DBA/2小鼠体内的P388D1细胞中B23易位的影响。放线菌素D处理后，细胞中观察到B23易位呈剂量和时间依赖性。药物处理后30分钟内即可观察到易位。单次注射0.25mg/kg可实现持续至少1天的完全B23易位。剂量降低会导致持续时间较短的部分B23易位。这些结果表明，B23易位可能有助于监测动物体内的药物作用。
