Recule C, Hirtz P, Le Noc P, Croizé J
Laboratoire de Bactériologie, CHRU de Grenoble.
Pathol Biol (Paris). 1992 May;40(5):433-9.
A characteristic feature of imipenem-resistant strains of Pseudomonas aeruginosa is loss or decreased expression of the outer membrane protein (OMP) D2, whose molecular weight is 45 to 49 kDa. D2 was studied in 15 strains of P. aeruginosa with intermediate susceptibility or resistance to imipenem recovered from the sputum of 15 patients with cystic fibrosis. The OMP was extracted using Sarkosyl and separated by SDS-PAGE electrophoresis. Electrophoresis patterns were compared to those of reference strains 3B and 3C which are resistant and susceptible to imipenem, respectively. Expression of D2 was normal in three strains, weak or very weak in 11 strains and absent in one strain. For 12 strains, the alteration of the D2 protein was consistent with previous reports. However, the finding of normal D2 production in three strains is unusual and suggests the possible presence of another mechanism of resistance.
耐亚胺培南铜绿假单胞菌菌株的一个特征是外膜蛋白（OMP）D2缺失或表达减少，其分子量为45至49 kDa。对从15例囊性纤维化患者痰液中分离出的15株对亚胺培南中度敏感或耐药的铜绿假单胞菌进行了D2研究。使用十二烷基肌氨酸钠提取OMP，并通过SDS-PAGE电泳进行分离。将电泳图谱与分别对亚胺培南耐药和敏感的参考菌株3B和3C的图谱进行比较。D2在3株菌株中表达正常，在11株菌株中表达较弱或非常弱，在1株菌株中缺失。对于12株菌株，D2蛋白的改变与先前报道一致。然而，在3株菌株中发现D2产生正常是不寻常的，这表明可能存在另一种耐药机制。
