Barbosa H S, de Meirelles M de N
Departamento de Ultra-estrutura e Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil.
Parasitol Res. 1992;78(5):404-9. doi: 10.1007/BF00931696.
The presence of carbohydrate residues in the plasma membrane of normal and Trypanosoma cruzi-infected heart muscle cells was investigated cytochemically using ruthenium red, lanthanum nitrate, periodic acid-Schiff/thiocarbohydrazide/silver, and gold- and ferritin-lectin complexes. The study combined conventional electron microscopy with the new analytical technique of electron spectroscopic imaging (ESI). Galactosyl, mannosyl, and sialyl residues were detected in regions of host-cell plasma membrane that undergo interiorization together with the parasite. Lectin-binding sites were sometimes found to show a punctate or patchy distribution in the endocytic vacuole membrane. These findings suggest the that glycoconjugates cytochemically detected in the host-cell plasma membrane participate in the invasion of heart muscle cells by T. cruzi.
利用钌红、硝酸镧、过碘酸-希夫试剂/硫代 carbohydrazide/银以及金和铁蛋白凝集素复合物，通过细胞化学方法研究了正常心肌细胞和克氏锥虫感染的心肌细胞质膜中碳水化合物残基的存在情况。该研究将传统电子显微镜与新的电子光谱成像分析技术（ESI）相结合。在与寄生虫一起内化的宿主细胞质膜区域检测到了半乳糖基、甘露糖基和唾液酸残基。在胞吞泡膜中有时发现凝集素结合位点呈点状或斑片状分布。这些发现表明，在宿主细胞质膜中通过细胞化学检测到的糖缀合物参与了克氏锥虫对心肌细胞的侵袭。
