源自RNA连接酶脱氧核酶更快变体的结构-功能相关性。
Structure-function correlations derived from faster variants of a RNA ligase deoxyribozyme.
作者信息
Prior Tracey K, Semlow Daniel R, Flynn-Charlebois Amber, Rashid Imran, Silverman Scott K
机构信息
Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA.
出版信息
Nucleic Acids Res. 2004 Feb 11;32(3):1075-82. doi: 10.1093/nar/gkh263. Print 2004.
We previously reported the in vitro selection of several Mg2+-dependent deoxyribozymes (DNA enzymes) that synthesize a 2'-5' RNA linkage from a 2',3'-cyclic phosphate and a 5'-hydroxyl. Here we subjected the 9A2 deoxyribozyme to re-selection for improved ligation rate. We found two new DNA enzymes (7Z81 and 7Z48) that contain the catalytic core of 7Q10, a previously reported small deoxyribozyme that is unrelated in sequence to 9A2. A third new DNA enzyme (7Z101) is unrelated to either 7Q10 or 9A2. The new 7Z81 and 7Z48 DNA enzymes have ligation rates over an order of magnitude higher than that of 7Q10 itself and they have additional sequence elements that correlate with these faster rates. Our findings provide insight into structure-function relationships of catalytic nucleic acids.
我们之前报道了几种依赖Mg2+的脱氧核酶（DNA酶）的体外筛选，这些酶能从2',3'-环磷酸酯和5'-羟基合成2'-5' RNA连接。在此，我们对9A2脱氧核酶进行重新筛选以提高连接速率。我们发现了两种新的DNA酶（7Z81和7Z48），它们含有7Q10的催化核心，7Q10是之前报道的一种小脱氧核酶，其序列与9A2无关。第三种新的DNA酶（7Z101）与7Q10或9A2均无关。新的7Z81和7Z48 DNA酶的连接速率比7Q10本身高出一个数量级以上，并且它们具有与这些更快速率相关的额外序列元件。我们的发现为催化核酸的结构-功能关系提供了见解。
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