Rajaganapathi J, Kathiresan K, Singh T P
Center of Advanced Study in Marine Biology, Annamalai University, Parangipettai-608 502, Tamil Nadu, India.
Mar Biotechnol (NY). 2002 Oct;4(5):447-53. doi: 10.1007/s10126-002-0012-2.
The purple fluid of Bursatella leachii, already found to have anti-HIV activity, was selected and tested for purification and characterization of an anti-HIV protein. Only one fraction showed anti-HIV activity at the minimum inhibition concentration of 50 mg/ml. This purified anti-HIV protein has been named as "Bursatellanin-P" after the animal species Bursatella leachii. About 3 mg of the pure protein was obtained from 1000 ml of purple fluid. The anti-HIV activity increased by about 135-fold in the purified sample, as compared with the crude purple fluid. The purified protein, which showed anti-HIV activity, was a single unit with a molecular weight of 60 kDa. The protein was stable between pH 5.8 and 8.0. It lost its activity with heating at 60 degrees C for 10 minutes and also with extreme pH values of 2.0 or 10. The protein was resistant to digestion of proteinase K and mercaptoethanol.
已发现具有抗HIV活性的海兔（Bursatella leachii）紫色液体被选来进行抗HIV蛋白的纯化和特性研究测试。只有一个组分在最低抑制浓度为50mg/ml时显示出抗HIV活性。这种纯化的抗HIV蛋白根据海兔（Bursatella leachii）这一动物物种被命名为“海兔素-P”。从1000ml紫色液体中获得了约3mg的纯蛋白。与粗紫色液体相比，纯化样品中的抗HIV活性增加了约135倍。显示出抗HIV活性的纯化蛋白是一个分子量为60kDa的单一单元。该蛋白在pH 5.8至8.0之间稳定。在60℃加热10分钟以及在极端pH值2.0或10的情况下它会失去活性。该蛋白对蛋白酶K和巯基乙醇的消化具有抗性。
