Dhahri Manel, Sioud Salim, Dridi Rihab, Hassine Mohsen, Boughattas Naceur A, Almulhim Fatimah, Al Talla Zeyad, Jaremko Mariusz, Emwas Abdul-Hamid M
Biology Department, Faculty of Science Yanbu, Taibah University, 46423 Yanbu El-Bahr, Saudi Arabia.
Analytical Core Lab, King Abdullah University of Science and Technology (KAUST), 23955-6900 Thuwal, Kingdom of Saudi.
ACS Omega. 2020 Jun 10;5(24):14786-14795. doi: 10.1021/acsomega.0c01724. eCollection 2020 Jun 23.
Bioactive compounds for drug discovery are increasingly extracted and purified from natural sources including marine organisms. Heparin is a therapeutic agent that has been used for several decades as an anticoagulant. However, heparin is known to cause many undesirable complications such as thrombocytopenia and risk of hemorrhage. Hence, there is a need to find alternatives to current widely used anticoagulant drugs. Here, we extract a sulfated polysaccharide from sea hare, that is, viscera, by enzymatic digestion. Several analytical approaches including elemental analysis, Fourier-transform infrared spectroscopy, nuclear magnetic resonance, and high-performance liquid chromatography-mass spectrometry analysis show that polysaccharides have chemical structures similar to glycosaminoglycans. We explore the anticoagulant activity of the extract using the activated partial thromboplastin time and the thrombin time. Our results demonstrate that the extracted sulfated polysaccharide has heparin-like anticoagulant activity, thus showing great promise as an alternative anticoagulant therapy.
用于药物研发的生物活性化合物越来越多地从包括海洋生物在内的天然来源中提取和纯化。肝素是一种已经作为抗凝剂使用了几十年的治疗剂。然而，肝素已知会引起许多不良并发症，如血小板减少症和出血风险。因此，有必要寻找当前广泛使用的抗凝药物的替代品。在此，我们通过酶消化从海兔的内脏中提取了一种硫酸化多糖。包括元素分析、傅里叶变换红外光谱、核磁共振和高效液相色谱 - 质谱分析在内的几种分析方法表明，该多糖具有与糖胺聚糖相似的化学结构。我们使用活化部分凝血活酶时间和凝血酶时间来探究提取物的抗凝活性。我们的结果表明，提取的硫酸化多糖具有类似肝素的抗凝活性，因此作为一种替代抗凝疗法显示出巨大的前景。
