Brenner V, Rucká L, Totevová S, Tømeraas K, Demnerová K
Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
J Appl Microbiol. 2004;96(3):430-6. doi: 10.1111/j.1365-2672.2004.02075.x.
A possibility for the complementation of both ortho- and meta-cleavage pathway for chlorocatechols in one strain and its impact on degradation of chlorobenzoates accumulated during degradation of polychlorinated biphenyls was investigated.
Genes responsible for ortho-cleavage of chlorocatechols were subcloned into two biphenyl degraders and the activities of chlorocatechol dioxygenases responsible for ortho- and meta-cleavage in these hybrid strains were monitored spectrophotometrically and also electrochemically by ion-selective electrode.
While strain Pseudomonas fluorescens S12/C apparently gained metabolic advantage from this gene manipulation, strain Burkholderia cepacia P166/C did not express better degradation features in comparison with the parental strain.
This approach has the potential to enhance chlorocatechol metabolism in selected biphenyl degraders.
研究在一个菌株中实现氯代儿茶酚邻位和间位裂解途径互补的可能性及其对多氯联苯降解过程中积累的氯代苯甲酸酯降解的影响。
将负责氯代儿茶酚邻位裂解的基因亚克隆到两种联苯降解菌中，并通过分光光度法以及离子选择性电极电化学法监测这些杂交菌株中负责邻位和间位裂解的氯代儿茶酚双加氧酶的活性。
虽然荧光假单胞菌S12/C菌株显然从这种基因操作中获得了代谢优势，但洋葱伯克霍尔德菌P166/C菌株与亲本菌株相比并未表现出更好的降解特性。
这种方法有可能增强选定联苯降解菌中氯代儿茶酚的代谢。
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