Martin Chaves E B, Brum I S, Stoll J, Capp E, Corleta H v E
Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
Gynecol Obstet Invest. 2004;57(4):210-3. doi: 10.1159/000076690. Epub 2004 Feb 9.
Uterine leiomyomas are the most common tumors of the genital tract. Growth factors seem to be implicated in the development of leiomyoma. The aim of this study was to determine insulin-like growth factor 1 receptor (IGF-1-R) mRNA levels and IGF-1-R tyrosine kinase activity in normal myometrium and leiomyoma. Plasma membranes of myometrium and leiomyoma of 14 women subjected to hysterectomy were prepared, and samples were incubated in the absence or presence of recombinant human IGF-1 to assess the tyrosine kinase activity (Western blot). Reverse-transcriptase polymerase chain reaction with specific primers for IGF-1-R was used to determine IGF-1-R mRNA levels. IGF-1-R mRNA levels in myometrium (0.8216 +/- 0.096) and in leiomyoma (0.7905 +/- 0.136) were not statistically significantly different (p = 0.648). The degree of IGF-1-R autophosphorylation stimulated by recombinant IGF-1 was not different in myometrium (1.020 +/- 0.120) and leiomyoma (1.620 +/- 0.656) either (p = 0.075). There was no difference in IGF-1-R expression and IGF-1-R autophosphorylation between normal myometrium and leiomyoma.
子宫平滑肌瘤是生殖道最常见的肿瘤。生长因子似乎与平滑肌瘤的发生有关。本研究的目的是测定正常子宫肌层和平滑肌瘤中胰岛素样生长因子1受体（IGF-1-R）mRNA水平及IGF-1-R酪氨酸激酶活性。制备了14例行子宫切除术妇女的子宫肌层和平滑肌瘤的质膜，样本在不存在或存在重组人IGF-1的情况下孵育以评估酪氨酸激酶活性（蛋白质免疫印迹法）。使用针对IGF-1-R的特异性引物进行逆转录聚合酶链反应来测定IGF-1-R mRNA水平。子宫肌层（0.8216±0.096）和平滑肌瘤（0.7905±0.136）中的IGF-1-R mRNA水平无统计学显著差异（p = 0.648）。重组IGF-1刺激的IGF-1-R自身磷酸化程度在子宫肌层（1.020±0.120）和平滑肌瘤（1.620±0.656）中也无差异（p = 0.075）。正常子宫肌层和平滑肌瘤之间的IGF-1-R表达及IGF-1-R自身磷酸化无差异。
