Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, People's Republic of China.
Gynecol Endocrinol. 2009 Aug;25(8):520-3. doi: 10.1080/09513590902972117.
It has been suggested that progesterone down-regulated the levels of insulin-like growth factor-I (IGF-I). The present study was conducted to elucidate whether progesterone affects IGF-I mRNA through progesterone receptor B(PRB) mRNA in leiomyoma.
Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to measure the level of progesterone receptor A (PRA), PRB, and IGF-I mRNA in 21 patients with uterine leiomyoma, and six pairs of matching leiomyomas and adjacent myometria were obtained in the secretory phase.
The ratios of PRA/PRB mRNA were positively correlated with the IGF-I mRNA levels in leiomyoma from women in the secretory phase (r = 0.732, p = 0.002 < 0.01), whereas the correlation between the level of PRB and IGF-I mRNA in leiomyomas was negative (r = -0.573, p = 0.025 < 0.05). The levels of PRA, PRB, and IGF-I mRNA were significantly higher in leiomyomas than in adjacent myometria.
The progesterone may down-regulate IGF-I expression through PRB, and PRA appears to inhibit this function of progesterone by inhibiting the transcription of PRB; thus, the action of progesterone on leiomyoma growth may depend on different ratios of PRA and PRB.
有研究表明孕激素可下调胰岛素样生长因子-I（IGF-I）的水平。本研究旨在探讨孕激素是否通过孕激素受体 B（PRB）mRNA 影响子宫肌瘤中的 IGF-I mRNA。
采用半定量反转录聚合酶链反应（RT-PCR）检测 21 例分泌期子宫肌瘤患者及 6 对配对子宫肌瘤和邻近子宫肌组织中孕激素受体 A（PRA）、PRB 和 IGF-I mRNA 的水平。
分泌期子宫肌瘤中 PRA/PRB mRNA 比值与 IGF-I mRNA 水平呈正相关（r = 0.732，p = 0.002 < 0.01），而 PRB 与 IGF-I mRNA 水平呈负相关（r = -0.573，p = 0.025 < 0.05）。子宫肌瘤中 PRA、PRB 和 IGF-I mRNA 的水平明显高于邻近子宫肌组织。
孕激素可能通过 PRB 下调 IGF-I 的表达，而 PRA 可能通过抑制 PRB 的转录来抑制孕激素的这一功能；因此，孕激素对子宫肌瘤生长的作用可能取决于 PRA 和 PRB 的不同比值。
