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A protein kinase substrate identified by the two-hybrid system.

作者信息

Yang X, Hubbard E J, Carlson M

机构信息

Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032.

出版信息

Science. 1992 Jul 31;257(5070):680-2. doi: 10.1126/science.1496382.

DOI:10.1126/science.1496382
PMID:1496382
Abstract

A genetic method, the two-hybrid system, was used to identify four genes encoding proteins that interact with the SNF1 protein kinase from yeast. One of the genes, SIP1, was independently isolated as a multicopy suppressor of defects caused by reduced SNF1 kinase activity, and genetic evidence supports its function in the SNF1 pathway. The SIP1 protein co-immunoprecipitated with SNF1 and was phosphorylated in vitro. Thus, the two-hybrid system, which is applicable to any cloned gene, can be used to detect physical interactions between protein kinases and functionally related substrate proteins.

摘要

相似文献

1
A protein kinase substrate identified by the two-hybrid system.
Science. 1992 Jul 31;257(5070):680-2. doi: 10.1126/science.1496382.
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The Snf1 protein kinase and its activating subunit, Snf4, interact with distinct domains of the Sip1/Sip2/Gal83 component in the kinase complex.Snf1蛋白激酶及其激活亚基Snf4与激酶复合物中Sip1/Sip2/Gal83组分的不同结构域相互作用。
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