Schindler C, Shuai K, Prezioso V R, Darnell J E
Laboratory of Molecular Cell Biology, Rockefeller University, New York, NY 10021.
Science. 1992 Aug 7;257(5071):809-13. doi: 10.1126/science.1496401.
The interferon-alpha (IFN-alpha)-stimulated gene factor 3 (ISGF3), a transcriptional activator, contains three proteins, termed ISGF3 alpha proteins, that reside in the cell cytoplasm until they are activated in response to IFN-alpha. Treatment of cells with IFN-alpha caused these three proteins to be phosphorylated on tyrosine and to translocate to the cell nucleus where they stimulate transcription through binding to IFN-alpha-stimulated response elements in DNA. IFN-gamma, which activates transcription through a different receptor and different DNA binding sites, also caused tyrosine phosphorylation of one of these proteins. The ISGF3 alpha proteins may be substrates for one or more kinases activated by ligand binding to the cell surface and may link occupation of a specific polypeptide receptor with activation of transcription of a set of specific genes.
干扰素α（IFN-α）刺激基因因子3（ISGF3）是一种转录激活因子，由三种蛋白质组成，称为ISGF3α蛋白，它们存在于细胞质中，直到在对IFN-α的反应中被激活。用IFN-α处理细胞会导致这三种蛋白质在酪氨酸上磷酸化，并转移到细胞核中，在那里它们通过与DNA中的IFN-α刺激反应元件结合来刺激转录。IFN-γ通过不同的受体和不同的DNA结合位点激活转录，也会导致这些蛋白质之一的酪氨酸磷酸化。ISGF3α蛋白可能是一种或多种因配体与细胞表面结合而激活的激酶的底物，并且可能将特定多肽受体的占据与一组特定基因的转录激活联系起来。
