Sechi L A, Mura M, Tanda E, Lissia A, Fadda G, Zanetti S
Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli Studi, Sassari, Italy.
New Microbiol. 2004 Jan;27(1):75-7.
Crohn's disease is a non-specific chronic transmural inflammatory disease. The disease was associated with a frameshit mutation in the NOD2 gene. Nevertheless, other researchers associated the presence of M. paratuberculosis within the intestinal tissues of patients with the disease. An adapted "in situ hybridization" technique was used to detect IS900 M. paratuberculosis DNA in paraffin embedded tissue from Crohns tissue disease samples. We were able to identify M. paratuberculosis DNA in around 69% of the paraffine embedded intestinal samples of Crohn's disease patients analysed. The presence of M. paratuberculosis DNA in the intestinal samples analysed does not necessarily mean that M. paratuberculosis is responsible for Crohn's disease. Our results support the hypothesis that infection may be caused by cell wall defective M. paratuberculosis since no bacteria were detected by Ziehl Neelsen stain.
克罗恩病是一种非特异性慢性透壁性炎症性疾病。该疾病与NOD2基因的移码突变有关。然而，其他研究人员将副结核分枝杆菌在该病患者肠道组织中的存在与之关联。一种改良的“原位杂交”技术被用于检测来自克罗恩病组织样本的石蜡包埋组织中的副结核分枝杆菌IS900 DNA。在分析的克罗恩病患者约69%的石蜡包埋肠道样本中，我们能够鉴定出副结核分枝杆菌DNA。在所分析的肠道样本中副结核分枝杆菌DNA的存在并不一定意味着副结核分枝杆菌是克罗恩病的病因。我们的结果支持这样的假说，即感染可能由细胞壁缺陷型副结核分枝杆菌引起，因为萋-尼染色未检测到细菌。
