Lambert C J, Egan T M
Division of Cardiothoracic Surgery, University of North Carolina School of Medicine, Chapel Hill 27599.
Transplantation. 1992 Aug;54(2):205-9. doi: 10.1097/00007890-199208000-00003.
The optimal timing of administration of free radical scavengers in lung preservation has yet to be determined. In previous studies, the oxygen free radical scavenger dimethylthiourea (DMTU) has improved pulmonary function when added to Eurocollins flush solution at the time of lung harvest and infused at the time of lung reimplantation. To determine when DMTU must be administered in order to preserve lung function most effectively, 16 dogs underwent lung allotransplantation. Donor lungs were flushed with modified Eurocollins solution (50 ml/kg) and stored for 12 hr at 4 degrees C. DMTU was either added to the flush solution (5 g) or infused (20 g over 2 hr) at the time of reimplantation. Investigators were blinded to the time of DMTU administration. The contralateral pulmonary artery was ligated after a 1-hr stabilization period. Measurements were recorded for 8 hr while keeping FiO2 constant at 40%. The flush group demonstrated significantly improved survival and oxygenation compared with the infusion group. The mortality rate was 50% in the infusion treatment group, while no deaths occurred in the flush animals. Lung function is adequately preserved after 12-hr storage when DMTU is administered in the flush solution at the time of harvest. It would appear that oxygen free radical scavengers must be present in lung tissue during storage in order to be effective.
自由基清除剂在肺保存中的最佳给药时机尚未确定。在先前的研究中，氧自由基清除剂二甲基硫脲（DMTU）在肺获取时添加到Eurocollins冲洗液中，并在肺再植入时输注，可改善肺功能。为了确定何时必须给予DMTU才能最有效地保护肺功能，16只狗接受了肺同种异体移植。供体肺用改良的Eurocollins溶液（50 ml/kg）冲洗，并在4℃下保存12小时。DMTU要么添加到冲洗液中（5 g），要么在再植入时输注（2小时内输注20 g）。研究人员对DMTU给药时间不知情。在1小时稳定期后结扎对侧肺动脉。在将FiO2保持在40%恒定的情况下记录8小时的测量值。与输注组相比，冲洗组的生存率和氧合情况有显著改善。输注治疗组的死亡率为50%，而冲洗组动物无死亡发生。当在收获时将DMTU添加到冲洗液中时，肺功能在储存12小时后得到充分保存。似乎氧自由基清除剂在储存期间必须存在于肺组织中才能有效。
