Detterbeck F C, Keagy B A, Paull D E, Wilcox B R
Division of Cardiothoracic Surgery, University of North Carolina School of Medicine, Chapel Hill.
Ann Thorac Surg. 1990 Aug;50(2):204-9; discussion 209-10. doi: 10.1016/0003-4975(90)90735-o.
An in vivo canine model was used to assess the ability of an oxygen free radical scavenger to decrease reperfusion injury in lung transplantation. In 12 dogs, the left lungs were transplanted after they had been preserved for 24 hours at 4 degrees C after pulmonary artery flushing with modified Eurocollins solution. In 6 dogs, dimethylthiourea, a potent oxygen free radical scavenger, was added to the flush solution and was also given to the recipients just before reperfusion. In all animals, the contralateral pulmonary artery and bronchus were ligated and lung function was assessed for 12 hours or until death. Three dogs died prematurely in the control group, whereas only 1 dog died prematurely in the dimethylthiourea group. This resulted in a statistically significant difference in the average length of survival (p less than 0.05). Pulmonary artery and right atrial pressures were significantly lower in the dimethylthiourea group during the first 6 hours (p less than 0.05). Treatment with dimethylthiourea resulted in a significantly higher arterial oxygen tension at 4 hours, and intrapulmonary shunt tended to be lower. Thus, it would appear that dimethylthiourea has a protective effect on lungs preserved for 24 hours before transplantation in dogs.
采用犬体内模型评估氧自由基清除剂减轻肺移植中再灌注损伤的能力。12只犬，左肺用改良的Eurocollins溶液经肺动脉冲洗后在4℃保存24小时，然后进行移植。6只犬，在冲洗液中加入强效氧自由基清除剂二甲基硫脲，并在再灌注前给予受体。所有动物，对侧肺动脉和支气管结扎，评估肺功能12小时或直至死亡。对照组3只犬过早死亡，而二甲基硫脲组只有1只犬过早死亡。这导致平均存活时间有统计学显著差异（p<0.05）。二甲基硫脲组在前6小时肺动脉和右心房压力显著更低（p<0.05）。二甲基硫脲治疗导致4小时时动脉血氧张力显著更高，肺内分流倾向更低。因此，似乎二甲基硫脲对犬移植前保存24小时的肺有保护作用。
