Shan Guomin, Huang Huazhang, Stoutamire Donald W, Gee Shirley J, Leng Gabriele, Hammock Bruce D
Department of Entomology, University of California, Davis, California 95616, USA.
Chem Res Toxicol. 2004 Feb;17(2):218-25. doi: 10.1021/tx034220c.
The pyrethroids are one of the most heavily used insecticide classes in the world. It is important to develop sensitive and rapid analytical techniques for environmental monitoring and assessment of human exposure to these compounds. Because major pyrethroids contain a phenoxybenzyl group and phenoxybenzoic acid (PBA) is a common metabolite form or intermediate, PBA might be used as a biomarker of human exposure to pyrethroids. A sensitive and selective immunoassay for the common pyrethroid metabolite PBA was developed. Rabbits were immunized with 3-[4-(3-carboxyphenoxy)phenoxy] N-thyroglobulin ethylamine. All sera were screened against numerous coating antigens. The assay with the least interference and the best sensitivity was optimized and characterized. The average IC50 for free PBA was 1.65 ng/mL. No cross-reactivity was measured to parent pyrethroids and other metabolites. Urine matrix effects can be eliminated by simple dilution. Results from urine samples from exposed workers suggest that this PBA immunoassay might be suitable as a monitoring tool for human exposure to pyrethroids.
拟除虫菊酯是世界上使用最为广泛的杀虫剂类别之一。开发灵敏且快速的分析技术用于环境监测以及评估人类对这些化合物的接触情况至关重要。由于主要的拟除虫菊酯含有苯氧基苄基，且苯氧基苯甲酸（PBA）是一种常见的代谢产物形式或中间体，PBA 可能被用作人类接触拟除虫菊酯的生物标志物。针对常见的拟除虫菊酯代谢产物 PBA 开发了一种灵敏且具选择性的免疫测定法。用 3-[4-(3-羧基苯氧基)苯氧基]N-甲状腺球蛋白乙胺对兔子进行免疫。所有血清均针对多种包被抗原进行筛选。对干扰最小且灵敏度最佳的测定法进行了优化和表征。游离 PBA 的平均半数抑制浓度（IC50）为 1.65 纳克/毫升。未检测到与母体拟除虫菊酯及其他代谢产物的交叉反应。通过简单稀释可消除尿液基质效应。来自接触工人尿液样本的结果表明，这种 PBA 免疫测定法可能适合作为监测人类接触拟除虫菊酯的工具。
