Konstantinova N A, Matveeva N A, Sirko I V, Firsov N N
Russian State Medical University, Moscow.
Clin Hemorheol Microcirc. 2004;30(1):25-32.
Nephelometry technique was used to register the alterations of the scattering signal from a whole blood sample due to erythrocyte aggregates in stasis and under controlled shear stress. The measured parameters were: the characteristic times of linear and three-dimensional aggregates formation, and the strength of aggregates of different types. These parameters depend on the sample temperature in the range of 2/50 degrees C. Temporal parameters of the aggregation process strongly increase (by 3 times) at temperature 45 degrees C. For samples of normal blood the aggregates strength parameters do not significantly depend on the sample temperature, whereas for blood samples from patients with cryoglobulinemia high increase of the strength of both three-dimensional and linear aggregates and decrease of time of linear aggregates formation at low temperature of the sample (4 degrees C) was observed. The difference of these parameters of the pathological blood from that of the normal at room temperature was quite opposite. Possible reasons of such behavior of aggregation state of blood and explanation of the observed effects are suggested.
采用比浊法技术记录全血样本在血液淤滞和可控剪切应力作用下因红细胞聚集而产生的散射信号变化。测量参数包括：线性和三维聚集体形成的特征时间，以及不同类型聚集体的强度。这些参数在2/50摄氏度范围内取决于样本温度。在45摄氏度时，聚集过程的时间参数大幅增加（增加3倍）。对于正常血液样本，聚集体强度参数在很大程度上不依赖于样本温度，而对于冷球蛋白血症患者的血液样本，在样本低温（4摄氏度）时，观察到三维和线性聚集体的强度显著增加，线性聚集体形成时间减少。在室温下，病理血液与正常血液这些参数的差异正好相反。文中提出了血液聚集状态出现这种行为的可能原因，并对观察到的效应进行了解释。
