用于诊断产气荚膜梭菌肠毒血症的酶联免疫吸附测定法的评估

Evaluation of enzyme-linked immunosorbent assay for diagnosis of Clostridium perfringens enterotoxemias.

作者信息

el Idrissi A H, Ward G E

机构信息

Département de Microbiologie et Maladies Contagieuses, Institut Agronomique et Vétérinaire Hassan II, Rabat, Morocco.

出版信息

Vet Microbiol. 1992 Jun 15;31(4):389-96. doi: 10.1016/0378-1135(92)90131-c.

DOI:10.1016/0378-1135(92)90131-c

PMID:1496812

Abstract

Two double sandwich enzyme-linked immunosorbent assays (ELISA) for Clostridium perfringens beta and epsilon toxins were assessed for routine diagnosis of enterotoxemias on intestinal contents of 151 sheep that died suddenly. Conventional tests (mouse assay and culture of organism) showed that 21 specimens were positive for Clostridium perfringens type C (beta toxin) and 39 were positive for Clostridium perfringens type D (epsilon toxin) enterotoxemias. Comparison of the ELISA results with conventional assays gave sensitivity and specificity rates respectively of 90.5% and 89.2% for beta toxin assay and 97.4% and 94.6% for epsilon toxin assay. With further refinement to improve the performance of the assay for beta toxin these tests could serve as a substitute for conventional tests in the laboratory diagnosis of Clostridium perfringens types B, C and D enterotoxemias.

摘要

对用于检测产气荚膜梭菌β毒素和ε毒素的两种双夹心酶联免疫吸附测定（ELISA）进行了评估，以用于对151只突然死亡绵羊的肠道内容物进行肠毒血症的常规诊断。传统检测方法（小鼠试验和细菌培养）表明，21份样本的产气荚膜梭菌C型（β毒素）呈阳性，39份样本的产气荚膜梭菌D型（ε毒素）肠毒血症呈阳性。ELISA结果与传统检测方法的比较显示，β毒素检测的灵敏度和特异性分别为90.5%和89.2%，ε毒素检测的灵敏度和特异性分别为97.4%和94.6%。随着对β毒素检测方法性能的进一步改进，这些检测可替代传统检测方法用于产气荚膜梭菌B、C和D型肠毒血症的实验室诊断。