用酶联免疫吸附测定法检测产气荚膜梭菌α毒素

Detection of Clostridium perfringens alpha toxin by enzyme-linked immunosorbent assay.

作者信息

Naylor R D, Martin P K, Barker L T

机构信息

Veterinary Investigation Centre, Harlescott, Shrewsbury.

出版信息

Res Vet Sci. 1997 Jul-Aug;63(1):101-2. doi: 10.1016/s0034-5288(97)90168-5.

DOI:10.1016/s0034-5288(97)90168-5

PMID:9368967

Abstract

An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of Clostridium perfringens alpha toxin in intestinal contents of animals which have died of suspected C perfringens type A enterotoxaemia. The test can also be used for testing culture supernatants of C perfringens isolates for the presence of alpha toxin. The test was sensitive and quantitative detecting toxin down to the 25ng level. The use of the ELISA for the detection of alpha toxin in conjunction with those for epsilon toxin and beta toxin, allows the differential diagnosis of C perfringens types A, B, C and D enterotoxaemias from samples of intestinal contents and the typing of cultures of C perfringens.

摘要

已开发出一种酶联免疫吸附测定（ELISA）法，用于检测死于疑似A型产气荚膜梭菌肠毒血症动物的肠道内容物中的产气荚膜梭菌α毒素。该检测方法也可用于检测产气荚膜梭菌分离株培养上清液中是否存在α毒素。该检测方法灵敏且可定量，能检测低至25纳克水平的毒素。将ELISA法用于检测α毒素，再结合检测ε毒素和β毒素的方法，可对取自肠道内容物样本的A型、B型、C型和D型产气荚膜梭菌肠毒血症进行鉴别诊断，并对产气荚膜梭菌培养物进行分型。