牛乳腺组织外植体培养物中细菌病原体的黏附和定植
Adherence and colonization by bacterial pathogens in explant cultures of bovine mammary tissue.
作者信息
Thomas L H, Leigh J A, Bland A P, Cook R S
机构信息
Institute for Animal Health, Newbury, Berkshire, UK.
出版信息
Vet Res Commun. 1992;16(2):87-96. doi: 10.1007/BF01839005.
Explant cultures of bovine mammary tissue taken from virgin heifers were used to examine adherence, colonization and cytopathogenesis of Streptococcus uberis, Streptococcus agalactiae, Streptococcus dysgalactiae, Staphylococcus aureus and Escherichia coli in the putative target tissue. None of the five bacteria was able to adhere to healthy ductular epithelium but all showed a marked tropism for exposed connective tissue. S. aureus and E. coli induced a marked cytopathic effect in ductular epithelium after 6 hours in culture but the bacteria were not in close association with the affected tissue. No evidence could be found to support the hypothesis that adherence to epithelium might be the first stage in the pathogenesis of mastitis caused by these organisms.
取自未孕小母牛的牛乳腺组织外植体培养物，用于检测乳房链球菌、无乳链球菌、停乳链球菌、金黄色葡萄球菌和大肠杆菌在假定靶组织中的黏附、定植及细胞致病作用。这五种细菌均无法黏附至健康的导管上皮，但均对暴露的结缔组织表现出明显的嗜性。金黄色葡萄球菌和大肠杆菌在培养6小时后，可在导管上皮中诱导明显的细胞病变效应，但细菌与受影响组织并无紧密关联。未发现证据支持上皮黏附可能是这些微生物引起乳腺炎发病机制的第一阶段这一假说。
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