Frost A J, Wanasinghe D D, Woolcock J B
Infect Immun. 1977 Jan;15(1):245-53. doi: 10.1128/iai.15.1.245-253.1977.
Some parameters affecting the adherence of microbes to the ductular epithelium of the bovine mammary gland were studied. Adherence increased from teat sinus to lactiferous sinus to the large ducts, and cells from the lactiferous sinus to the large ducts, and cells from the lactiferous sinus were used for all other experiments. There was no difference in adherence to cells from different quarters of the same cow, but there were significant differences between cows. Scanning electron microscopy suggested that the cells of the ductular epithelium undergo dynamic changes that probably result in secretion and/or desquamation. Adherence to cells could be demonstrated only at a late stage of these changes. The adherence of organisms associated with mastitis was studied using an in vitro test. Adherence generally paralleled prevalence as cause of disease, with Staphylococcus aureus and Streptococcus agalactiae adhering best. Strain variation suggested that virulence was related to adherence with S. agalactiae and S. dysgalactiae but not with S. aureus. It is proposed that specific adherence is an important aspect of pathogenesis of mastitis due to S. aureus and S. agalactiae.
研究了一些影响微生物黏附于牛乳腺小叶上皮的参数。黏附从乳头窦到输乳窦再到大导管逐渐增加，后续所有实验均采用输乳窦及大导管的细胞。同一头牛不同乳腺区的细胞在黏附方面无差异，但不同奶牛之间存在显著差异。扫描电子显微镜显示，小叶上皮细胞会发生动态变化，这可能导致分泌和/或脱屑。只有在这些变化的后期才能证明微生物对细胞的黏附。采用体外试验研究了与乳腺炎相关的微生物的黏附情况。黏附情况总体上与疾病病因的流行程度平行，其中金黄色葡萄球菌和无乳链球菌的黏附性最强。菌株差异表明，无乳链球菌和停乳链球菌的毒力与黏附有关，而金黄色葡萄球菌则无关。研究表明，特异性黏附是金黄色葡萄球菌和无乳链球菌引起乳腺炎发病机制的一个重要方面。
