Polikarpov N A, Bevz N I, Viktorov A N, Liannaia A M, Kiseleva I A
Zh Mikrobiol Epidemiol Immunobiol. 1992 Apr(4):6-8.
The possibility of the formation of exoenzymes, such as DNAase, RNAase and hemolysin, by bifidobacteria was studied in comparison with their acid-forming and adhesive activity. Bifidobacterium reference strains, originally isolated from healthy adults and children, were studied. The study involved altogether 73 strains of bifidobacteria, including 24 B. bifidum strains, 13 B. adolescentis strains, 7 B. infantis strains, 10 B. breve strains and 19 B. longum strains. The bifidobacteria under study were shown to differ not only in the presence and activity of properties useful for macroorganisms, but also in the presence of enzymes having depolymerizing activity (DNAase, hemolysin). Thus, out of 73 strains under study 9 proved to be DNAase-positive and 6, hemolysin positive. At the same time a specific feature of bifidobacteria was their high acid-forming activity with the complete absence of RNAase activity and insignificant DNAase- and hemolysin-forming activity.
研究了双歧杆菌产生外切酶（如脱氧核糖核酸酶、核糖核酸酶和溶血素）的可能性，并与其产酸和黏附活性进行了比较。研究了最初从健康成人和儿童中分离出的双歧杆菌参考菌株。该研究共涉及73株双歧杆菌，包括24株双歧双歧杆菌、13株青春双歧杆菌、7株婴儿双歧杆菌、10株短双歧杆菌和19株长双歧杆菌。所研究的双歧杆菌不仅在对大型生物有用的特性的存在和活性方面存在差异，而且在具有解聚活性的酶（脱氧核糖核酸酶、溶血素）的存在方面也存在差异。因此，在所研究的73株菌株中，有9株被证明是脱氧核糖核酸酶阳性，6株是溶血素阳性。同时，双歧杆菌的一个特点是它们具有高的产酸活性，完全没有核糖核酸酶活性，脱氧核糖核酸酶和溶血素形成活性也不显著。
