Berrang M E, Smith D P, Windham W R, Feldner P W
US Department of Agriculture-Agricultural Research Service, PO Box 5677, Athens, Georgia 30604-5677, USA.
J Food Prot. 2004 Feb;67(2):235-8. doi: 10.4315/0362-028x-67.2.235.
Intestinal contents may contaminate broiler carcasses during processing. The objective of this study was to determine what effect various levels of intestinal contents had on the numbers of Campylobacter detected in broiler carcass rinse samples. Eviscerated broiler carcasses were collected from the shackle line in a commercial processing plant immediately after passing through an inside/outside washer. Broiler carcasses were cut longitudinally into contralateral halves using a sanitized saw. Cecal contents from the same flock were collected, pooled, homogenized, and used to contaminate carcass halves. Paired carcass halves were divided into groups of eight each, and then cecal contents (2, 5, 10, 50, or 100 mg) were placed onto one randomly selected half of each carcass, while the corresponding half of the same broiler carcass received no cecal contents. Campylobacter counts from carcass halves with cecal contamination were compared to the uncontaminated halves of the same carcasses using a paired t test. Carcass halves with 5 mg or more of surface cecal contamination had significantly higher numbers of Campylobacter than those without (P < 0.01). Carcass halves contaminated with only 5 mg of cecal contents had an average of 3.3 log CFU Campylobacter per ml of rinse, while corresponding uncontaminated carcass halves had 2.6 log CFU Campylobacter per ml of rinse. These data indicate that even small (5 mg) amounts of cecal contents can cause a significant increase in the numbers of Campylobacter on eviscerated broiler carcasses. Therefore, it is important to keep such contamination to a minimum during processing.
在加工过程中，肠道内容物可能会污染肉鸡胴体。本研究的目的是确定不同水平的肠道内容物对肉鸡胴体冲洗样本中检测到的弯曲杆菌数量有何影响。从一家商业加工厂的挂钩线上收集经过内外清洗机后立即取出的去内脏肉鸡胴体。使用消毒锯将肉鸡胴体纵向切成对侧两半。收集来自同一鸡群的盲肠内容物，合并、匀浆后用于污染胴体的一半。将配对的胴体半片分成每组8个，然后将盲肠内容物（2、5、10、50或100毫克）放置在每个胴体随机选择的一半上，而同一肉鸡胴体的相应另一半不接受盲肠内容物。使用配对t检验比较有盲肠污染的胴体半片与同一胴体未受污染半片的弯曲杆菌计数。表面盲肠污染5毫克或更多的胴体半片弯曲杆菌数量显著高于未受污染的半片（P < 0.01）。仅被5毫克盲肠内容物污染的胴体半片每毫升冲洗液中弯曲杆菌的平均数量为3.3 log CFU，而相应未受污染的胴体半片每毫升冲洗液中有2.6 log CFU弯曲杆菌。这些数据表明，即使少量（5毫克）的盲肠内容物也会导致去内脏肉鸡胴体上的弯曲杆菌数量显著增加。因此，在加工过程中将此类污染降至最低很重要。
