Tsai Guo-Jane, Zhang Shu-Lin, Shieh Pei-Ling
Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan, Republic of China.
J Food Prot. 2004 Feb;67(2):396-8. doi: 10.4315/0362-028x-67.2.396.
A water-soluble chitosan hydrolysate with high activity against Escherichia coli was obtained during cellulase digestion of chitosan for 18 h. This 18-h hydrolysate is composed of low-molecular-weight chitosan (LMWC), with a molecular weight of 12.0 kDa, and chitooligosaccharides, which are composed of sugars with a degree of polymerization of 1 to 8. LMWC has a strong activity at 100 ppm against many pathogens and yeast species, including Bacillus cereus, E. coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica serovar Typhi, and Saccharomyces cerevisiae, while the chitooligosaccharides have much weaker antimicrobial activity than does LMWC. Accordingly, the antimicrobial activity against E. coli in the 18-h hydrolysate proved to come mainly from the presence of LMWC.
壳聚糖经纤维素酶消化18小时后，可得到一种对大肠杆菌具有高活性的水溶性壳聚糖水解产物。这种18小时的水解产物由分子量为12.0 kDa的低分子量壳聚糖（LMWC）和由聚合度为1至8的糖组成的壳寡糖组成。LMWC在100 ppm时对许多病原体和酵母种类具有很强的活性，包括蜡样芽孢杆菌、大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌、伤寒沙门氏菌和酿酒酵母，而壳寡糖的抗菌活性比LMWC弱得多。因此，18小时水解产物对大肠杆菌的抗菌活性主要来自LMWC的存在。
