Wang Xi, Plhak Leslie C
Department of Food Science, University of Wisconsin, Madison, Wisconsin 53706, USA.
J Agric Food Chem. 2004 Feb 25;52(4):709-12. doi: 10.1021/jf034926c.
Immunogens, prepared by conjugating either (+)-gossypol or (-)-gossypol to Limulus polyphemushemolymph protein, were used for immunization in the production of monoclonal antibodies. Hybridoma were evaluated for their relative affinity to racemic gossypol, (+)-gossypol, (-)-gossypol, gossypol analogues, and their lysine derivatives. The monoclonal antibody obtained showed higher affinity to gossypol and gossypol analogues as compared to their lysine derivative counterparts. An indirect competitive enzyme-linked immunosorbent assay (ELISA) with this antibody was used to measure gossypol in 15 cottonseed meal products; the results showed good correlation with results obtained using the AOCS (free gossypol) official method (R(2) = 0.89). The direct recognition of both free gossypol and bound gossypol using this antibody will be useful for rapid screening and quality control.
通过将（+）-棉酚或（-）-棉酚与鲎血淋巴蛋白偶联制备的免疫原，用于单克隆抗体生产中的免疫接种。评估杂交瘤对消旋棉酚、（+）-棉酚、（-）-棉酚、棉酚类似物及其赖氨酸衍生物的相对亲和力。与赖氨酸衍生物对应物相比，获得的单克隆抗体对棉酚和棉酚类似物表现出更高的亲和力。使用该抗体的间接竞争酶联免疫吸附测定（ELISA）来测量15种棉籽粕产品中的棉酚；结果显示与使用美国油脂化学家协会（游离棉酚）官方方法获得的结果具有良好的相关性（R² = 0.89）。使用该抗体直接识别游离棉酚和结合棉酚将有助于快速筛选和质量控制。
