Department of Agri-food Industry, Food and Nutrition (LAN), "Luiz de Queiroz" College of Agriculture (ESALQ), University of São Paulo (USP), Piracicaba, Brazil.
Animal Nutrition, Center for Nuclear Energy in Agriculture, University of São Paulo (USP), Piracicaba, Brazil.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2021 Apr;38(4):659-669. doi: 10.1080/19440049.2020.1865579. Epub 2021 Feb 4.
Free gossypol is a toxic compound which naturally occurs in cottonseed and its derivates, affecting animal and possibly human health. Consequently, alternatives for gossypol destruction must be evaluated. This work evaluated the emerging technology of ozone processing for free gossypol destruction in cottonseed meal. Ozonation was carried out in the actual cottonseed meal and also a model system, designed to describe the involved mode of action. The model system consisted of glass pearls beads covered with free gossypol. Ozonation was performed in two ways: as a static process, i.e., without homogenising the sample after placing them in the reactor, and also homogenising it. Ozone degraded free gossypol in all the systems, but reaching different levels. Free gossypol reduction was higher in the model system than the cottonseed meal, and higher in the homogenised processing than the static one: cottonseed meal in homogenised (56%) and static (25%); model system homogenised (98%) and static (80%). The obtained differences suggest a problem of gas penetration in the solid particles, the effect of unexposed surfaces due to contact areas, and the reaction with other organic molecules further than the target. Ozonation is a promising technique for gossypol degradation in cottonseed meal, but additional strategies are needed to optimise the ozonation process and evaluate toxicological aspects.
游离棉酚是一种有毒化合物，天然存在于棉籽及其衍生物中，影响动物甚至人类的健康。因此，必须评估棉酚破坏的替代方法。本工作评估了臭氧处理技术在棉籽粕中游离棉酚破坏方面的应用。在实际棉籽粕和设计用于描述相关作用模式的模型体系中进行了臭氧化作用。模型体系由覆盖有游离棉酚的玻璃珠珠组成。臭氧化作用以两种方式进行：一种是静态过程，即在将样品放入反应器后不进行均化，另一种是进行均化。在所有体系中，臭氧均降解了游离棉酚，但达到的水平不同。模型体系中的游离棉酚减少量高于棉籽粕中的游离棉酚减少量，而在均化处理中的减少量高于静态处理中的减少量：棉籽粕中为均化（56%）和静态（25%）；模型体系中为均化（98%）和静态（80%）。所得差异表明存在气体在固体颗粒中的渗透问题、由于接触面积而导致未暴露表面的影响以及与目标以外的其他有机分子的反应。臭氧处理技术是一种有前途的棉酚降解技术，但需要额外的策略来优化臭氧化过程并评估毒理学方面。
