Liu Jun, Wang Zhan-Min, Zhen Shu-Fang, Wu Xiao-Peng, Ma Dao-Xin, Li Zhao-Hui, Liu Bo, Zhao Zhi-Lun, Ke Yang
Department of General Surgery, Qilu Hospital, Shandong University, Jinan 250012, China.
Hepatobiliary Pancreat Dis Int. 2004 Feb;3(1):110-4.
A diverse range of cytogenetic alterations of autosomal chromosomes has been reported to date. However, few studies have addressed the abnormalities of X chromosome in hepatocellular carcinoma (HCC) except sporadic reports on the deletion of band F1 in X chromosome, and the clonal analysis of methylation pattern of the X chromosome-linked human androgen receptor gene. Identification of specific X chromosome alterations during the course of neoplastic development would be essential to defining the genetic basis of HCC. Therefore, we studied the regularity of aberration of X chromosome in liver cancer.
Hepatocarcinoma cellular lines and tumor tissues were detected respectively through DNA probes of X chromosome after fluorescence in situ hybridization (FISH).
Increased copies of X chromosome were observed in all samples, and four signals of hybridization were of the major type.
Increased copy number of X chromosome frequently occur in liver cancer. The relationship between copy number of X chromosome and liver cancer genesis needs further investigation. This study is the first of its kind determining the copy number of X chromosome in liver cancer by using FISH.
