Feucht W, Treutter D, Christ E
Technical University of Munich, Faculty of Agriculture and Horticulture, D-8050 Freising-Weihenstephan, Germany.
Tree Physiol. 1992 Mar;10(2):169-77. doi: 10.1093/treephys/10.2.169.
The localization of catechins and proanthocyanidins in the phloem of elm (Ulmus minor Mill.) and cherry (Prunus avium L.) was determined histochemically by use of 4-dimethylaminocinnamaldehyde (DMACA) reagent. The two tree species showed a characteristic distribution of these phenols, which were most abundant in the phloem rays of cherry, but were largely confined to upright parenchyma cells of elm. Quantitative determination and qualitative separation of catechins and proanthocyanidins were performed using HPLC-CRD (chemical reaction detection), with 4-dimethylaminocinnamaldehyde as the staining reagent. Up to 14 different catechins and proanthocyanidins were found in both species.
通过使用4-二甲基氨基肉桂醛（DMACA）试剂，采用组织化学方法确定了儿茶素和原花青素在榆（小叶榆）和樱桃（欧洲甜樱桃）韧皮部中的定位。这两种树种显示出这些酚类物质的特征性分布，它们在樱桃的韧皮射线中含量最高，但在榆中主要局限于直立的薄壁细胞。使用HPLC-CRD（化学反应检测），以4-二甲基氨基肉桂醛作为染色试剂，对儿茶素和原花青素进行了定量测定和定性分离。在这两个树种中均发现了多达14种不同的儿茶素和原花青素。
