Alisky Joseph M, Davidson Beverly L
Marshfield Clinic Research Foundation, Marshfield, WI, USA.
Methods Mol Biol. 2004;246:91-120. doi: 10.1385/1-59259-650-9:91.
Recombinant adenoviral (Ad) vectors are derived from human adenoviruses: nonenveloped, encapsidated linear, double-stranded DNA viruses that commonly cause respiratory and gastrointestinal infections. Forty-three different human adenovirus serotypes have been characterized. Details about production of recombinant Ad vectors are given in Chapter 1. Ad vectors in widespread use are derived from human Ad serotypes 2 and 5 (Ad2 and Ad5), Ad5 being more common for applications in the central nervous system (CNS). Ad5 replication-impaired vectors most often contain deletions in the E1 and E3 regions, with transgenes driven by a variety of promoters including viral promoters, and those that are neuron-specific (2). Recently fiber-modified and "gutless" Ad vectors, and those based on canine adenovirus serotype 2, have been developed for use in brain (3-9).
重组腺病毒（Ad）载体源自人类腺病毒：无包膜、衣壳化的线性双链DNA病毒，通常引起呼吸道和胃肠道感染。已鉴定出43种不同的人类腺病毒血清型。第1章给出了重组Ad载体生产的详细信息。广泛使用的Ad载体源自人类Ad血清型2和5（Ad2和Ad5），Ad5在中枢神经系统（CNS）应用中更为常见。Ad5复制缺陷型载体最常包含E1和E3区域的缺失，转基因由多种启动子驱动，包括病毒启动子和神经元特异性启动子（2）。最近，已开发出纤维修饰的“无内脏”Ad载体以及基于犬腺病毒血清型2的载体用于脑部（3 - 9）。
