Mashima S
Department of Molecular Genetics, Laboratory of Racing Chemistry, Utsunomiya, Japan.
Cytogenet Genome Res. 2003;102(1-4):196-200. doi: 10.1159/000075748.
The MHC class II DQB gene of horse was isolated and characterized. No obvious mutations causing frame shifts, or destruction of putative protein structure and splicing machinery were detected. Nucleotide sequence of exon 2 was consistent with an allelic sequence of the W23 haplotype. The cytoplasmic region of the equine DQB gene comprised two exons and an intron. A novel fragment of the gene was identified at the 3' intergenic region proximal to the ELA-DQB gene by sequence comparison between the human and horse DQB genes. This sequence showed the highest identity to exon 3 region of the DQB gene, however the 5' half of this exon was truncated as compared with the intact exon. This gene fragment was also identified in the same site of the HLA-DQB gene.
对马的MHC II类DQB基因进行了分离和鉴定。未检测到导致移码、假定蛋白质结构破坏或剪接机制破坏的明显突变。外显子2的核苷酸序列与W23单倍型的等位基因序列一致。马DQB基因的胞质区由两个外显子和一个内含子组成。通过人与马DQB基因的序列比较，在ELA - DQB基因近端的3'基因间区域鉴定出该基因的一个新片段。该序列与DQB基因外显子3区域的同源性最高，但与完整外显子相比，该外显子的5'端一半被截断。在HLA - DQB基因的相同位点也鉴定出了该基因片段。
