Thomas R, Fiegler H, Ostrander E A, Galibert F, Carter N P, Breen M
Oncology Research Group, Centre for Preventive Medicine, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk, UK.
Cytogenet Genome Res. 2003;102(1-4):254-60. doi: 10.1159/000075758.
As with many human cancers, canine tumors demonstrate recurrent chromosome aberrations. A detailed knowledge of such aberrations may facilitate diagnosis, prognosis and the selection of appropriate therapy. Following recent advances made in human genomics, we are developing a DNA microarray for the domestic dog, to be used in the detection and characterization of copy number changes in canine tumors. As a proof of principle, we have developed a small-scale microarray comprising 87 canine BAC clones. The array is composed of 26 clones selected from a panel of 24 canine cancer genes, representing 18 chromosomes, and an additional set of clones representing dog chromosomes 11, 13, 14 and 31. These chromosomes were shown previously to be commonly aberrant in canine multicentric malignant lymphoma. Clones representing the sex chromosomes were also included. We outline the principles of canine microarray development, and present data obtained from microarray analysis of three canine lymphoma cases previously characterized using conventional cytogenetic techniques.
与许多人类癌症一样，犬类肿瘤也表现出反复出现的染色体畸变。详细了解这些畸变可能有助于诊断、预后评估以及选择合适的治疗方法。随着人类基因组学的最新进展，我们正在开发一种用于家犬的DNA微阵列，用于检测和表征犬类肿瘤中的拷贝数变化。作为原理验证，我们开发了一种包含87个犬类BAC克隆的小规模微阵列。该阵列由从一组24个犬类癌症基因中选出的26个克隆组成，这些基因代表18条染色体，另外还有一组代表犬类11号、13号、14号和31号染色体的克隆。这些染色体先前已被证明在犬类多中心恶性淋巴瘤中通常会发生畸变。还包括了代表性染色体的克隆。我们概述了犬类微阵列开发的原理，并展示了通过微阵列分析从先前使用传统细胞遗传学技术表征的三例犬类淋巴瘤病例中获得的数据。
