Menotti-Raymond M, David V A, Agarwala R, Schäffer A A, Stephens R, O'Brien S J, Murphy W J
Laboratory of Genomic Diversity, National Cancer Institute, Department of Health and Human Services, Frederick, MD 21702, USA.
Cytogenet Genome Res. 2003;102(1-4):272-6. doi: 10.1159/000075762.
Effective utilization of the domestic cat as an animal model for hereditary and infectious disease requires the development and implementation of high quality gene maps incorporating microsatellites and conserved coding gene markers. Previous feline linkage and radiation hybrid maps have lacked sufficient microsatellite coverage on all chromosomes to make effective use of full genome scans. Here we report the isolation and genomic mapping of 304 novel polymorphic repeat loci in the feline genome. The new loci were mapped in the domestic cat radiation hybrid panel using an automated fluorescent TAQ-Man based assay. The addition of these 304 microsatellites brings the total number of microsatellites mapped in the feline genome to 580, and the total number of loci placed onto the RH map to 1,126. Microsatellites now span every autosome with an average spacing of roughly one polymorphic STR every five centimorgans, and full genome coverage of one marker every 2.7 megabases. These loci now provide a useful tool for undertaking full-genome scans to identify genes associated with phenotypes of interest, such as those relating to hereditary disease, coat color, patterning and morphology. These resources can also be extended to the remaining 36 species of the cat family for population genetic and evolutionary genomic analyses.
要将家猫有效地用作遗传性和传染性疾病的动物模型，需要开发并实施包含微卫星和保守编码基因标记的高质量基因图谱。先前的猫科动物连锁图谱和辐射杂种图谱在所有染色体上缺乏足够的微卫星覆盖，无法有效利用全基因组扫描。在此，我们报告了猫科动物基因组中304个新的多态性重复位点的分离和基因组定位。使用基于荧光TAQ-Man的自动化检测方法，将这些新位点定位到家猫辐射杂种板中。这304个微卫星的加入使猫科动物基因组中定位的微卫星总数达到580个，定位到辐射杂种图谱上的位点总数达到1126个。现在，微卫星跨越了每一条常染色体，平均间距约为每五个厘摩有一个多态性短串联重复序列，全基因组覆盖范围为每2.7兆碱基有一个标记。这些位点现在为进行全基因组扫描以鉴定与感兴趣的表型相关的基因提供了一个有用的工具，例如那些与遗传性疾病、毛色、斑纹和形态有关的基因。这些资源还可以扩展到猫科的其余36个物种，用于群体遗传学和进化基因组学分析。
