Greve Torben, Callesen Henrik
Department of Clinical Studies, Reproduction, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
Reprod Fertil Dev. 2004;16(1-2):113-22. doi: 10.10371/RD03084.
The present review describes a range of selected farm animal embryo technologies used in embryological research and applied in animal breeding and production. Some of the techniques are driven by the breeder's wish to obtain animals with higher breeding values, whereas others are primarily driven by the curiosity of researchers. The interaction between basic research and practical application in these areas is still a characteristic feature for people who contribute to the International Embryo Transfer Society (IETS) and has been an advantage for both researchers and breeders. One example of such an interaction is that detailed structural analyses have described quality differences between embryos of various origins and, following embryo transfer, the pregnancy results have confirmed the correlation between morphology and viability. Another example is that polymerase chain reaction technology has allowed detection of Y-specific sequences in male embryos and has become a tool in animal production today. Data from domestic animal genome sequencing will provide a great deal of new information. A major challenge for the years to come will be using this information in a physiologically meaningful context and to continue the efforts to convert the laboratory experience into use in practise. Finally, it is important to obtain societal acceptance for a wider application of many of the technologies, such as in vitro embryo production and cloning.
本综述描述了一系列用于胚胎学研究、并应用于动物育种和生产的特定农场动物胚胎技术。其中一些技术是由育种者获得具有更高育种价值动物的愿望所驱动,而其他一些技术主要是由研究人员的好奇心所驱动。在这些领域,基础研究与实际应用之间的相互作用仍然是国际胚胎移植协会(IETS)成员的一个显著特征,并且对研究人员和育种者而言都是一项优势。这种相互作用的一个例子是,详细的结构分析描述了不同来源胚胎之间的质量差异,并且在胚胎移植后,妊娠结果证实了形态与活力之间的相关性。另一个例子是,聚合酶链反应技术已能够检测雄性胚胎中的Y特异性序列,并且如今已成为动物生产中的一种工具。家畜基因组测序数据将提供大量新信息。未来几年的一项重大挑战将是在具有生理意义的背景下利用这些信息,并继续努力将实验室经验转化为实际应用。最后,获得社会对许多技术(如体外胚胎生产和克隆)更广泛应用的认可非常重要。