Vandecasteele Stefaan J, Van Eldere Johan, Merckx Rita, Peetermans Willy E
Infectious Diseases Research Group, Rega Institute for Medical Research, University Leuven, Leuven, Belgium.
Diagn Microbiol Infect Dis. 2004 Feb;48(2):89-95. doi: 10.1016/j.diagmicrobio.2003.09.015.
Qualitative broth culture and quantitative culture on agar were compared with quantitative polymerase chain reaction (PCR) for the diagnosis of foreign body infections (FBI) in a rat model with and without exposure to systemic antibiotics (teicoplanin and rifampin). The 3 methods had a similar and high yield without antibiotics. Antibiotics decreased the number of CFU/foreign body and increased the number of culture-negative foreign bodies and the variability of the results in quantitative culture. The yield of broth culture remained high under antibiotics although prolonged incubation (2-5 days) was required. The yield of the PCR was equivalent or even superior (for teicoplanin) to the yield of broth culture. Quantitative PCR had a higher yield and lower variability than quantitative culture and was not affected by antibiotics. The simultaneous isolation of RNA from all samples indicated viability of the bacteria. Quantitative PCR seems a promising method for the diagnosis of FBI.
在有或没有全身性抗生素（替考拉宁和利福平）暴露的大鼠模型中，将定性肉汤培养和琼脂定量培养与定量聚合酶链反应（PCR）进行比较，以诊断异物感染（FBI）。在没有抗生素的情况下，这三种方法具有相似的高检出率。抗生素减少了每异物的CFU数量，增加了培养阴性异物的数量以及定量培养结果的变异性。尽管需要延长孵育时间（2 - 5天），但在抗生素作用下肉汤培养的检出率仍然很高。PCR的检出率与肉汤培养相当甚至更高（对于替考拉宁）。定量PCR比定量培养具有更高的检出率和更低的变异性，并且不受抗生素的影响。从所有样本中同时分离RNA表明细菌具有活力。定量PCR似乎是一种有前景的FBI诊断方法。
