Devi K N, Hemavathi B, Aliya S, Raveendra A, Thyagaraju K
Department of Biochemistry, SV University, Andhra Pradesh, India.
J Biochem Mol Biol Biophys. 2002 Dec;6(6):373-8. doi: 10.1080/1025814021000024299.
Glutathione S-transferases (GSTs; EC, 2.5.1.18) ubiquitously distributed in all life forms, are a family of multigene and multifunctional dimeric proteins, which play a main role in drug detoxication. On purification and on electrophoresis, rat testicular glutathione S-transferases showed that it comprises of four subunits, Yc of alpha class, Yb and Ybeta of mu class and Ydelta of pi class. On chromatofocusing they were resolved into six anionic and four cationic isozymes. The substrate specificity studies and immunoblot analysis of testis proteins revealed that Ydelta of pi class GST was induced predominantly in response to phenobarbitol and Yc of alpha class and Ybeta of mu class were elevated specifically on treatment with methylcholanthrene (MC). These results show that structural variation between the two carcinogens induces different types of GST subunits. Therefore, these subunits may be used as marker proteins for specific chemical toxicity of rat testis.
谷胱甘肽S-转移酶（GSTs；EC 2.5.1.18）普遍存在于所有生命形式中，是一个多基因且多功能的二聚体蛋白家族，在药物解毒过程中起主要作用。对大鼠睾丸谷胱甘肽S-转移酶进行纯化和电泳分析表明，它由四个亚基组成，分别是α类的Yc、μ类的Yb和Yβ以及π类的Yδ。通过层析聚焦，它们被分离为六种阴离子同工酶和四种阳离子同工酶。对睾丸蛋白的底物特异性研究和免疫印迹分析表明，π类GST的Yδ主要在苯巴比妥作用下被诱导，而α类的Yc和μ类的Yβ在经甲基胆蒽（MC）处理后特异性升高。这些结果表明，两种致癌物之间的结构差异诱导了不同类型的GST亚基。因此，这些亚基可作为大鼠睾丸特定化学毒性的标记蛋白。
