新月柄杆菌表面黏附的发育
Development of surface adhesion in Caulobacter crescentus.
作者信息
Bodenmiller Diane, Toh Evelyn, Brun Yves V
机构信息
Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.
出版信息
J Bacteriol. 2004 Mar;186(5):1438-47. doi: 10.1128/JB.186.5.1438-1447.2004.
Caulobacter crescentus has a dimorphic life cycle composed of a motile stage and a sessile stage. In the sessile stage, C. crescentus is often found tightly attached to a surface through its adhesive holdfast. In this study, we examined the contribution of growth and external structures to the attachment of C. crescentus to abiotic surfaces. We show that the holdfast is essential but not sufficient for optimal attachment. Rather, adhesion in C. crescentus is a complex developmental process. We found that the attachment of C. crescentus to surfaces is cell cycle regulated and that growth or energy or both are essential for this process. The initial stage of attachment occurs in swarmer cells and is facilitated by flagellar motility and pili. Our results suggest that strong attachment is mediated by the synthesis of a holdfast as the swarmer cell differentiates into a stalked cell.
新月柄杆菌具有由游动阶段和固着阶段组成的二态生命周期。在固着阶段，新月柄杆菌常常通过其粘性的柄细胞紧密附着于表面。在本研究中，我们研究了生长和外部结构对新月柄杆菌附着于非生物表面的作用。我们发现柄细胞对于最佳附着至关重要，但并不充分。相反，新月柄杆菌中的粘附是一个复杂的发育过程。我们发现新月柄杆菌附着于表面受细胞周期调控，并且生长或能量或两者对于该过程都是必不可少的。附着的初始阶段发生在游动细胞中，并由鞭毛运动和菌毛促进。我们的结果表明，当游动细胞分化为柄细胞时，牢固的附着是由柄细胞的合成介导的。
Zotero 插件